Publications

Peeling back the many layers of competitive exclusion.

Maurer JJ, Cheng Y, Pedroso A, Thompson KK, Akter S, Kwan T, Morota G, Kinstler S, Porwollik S, McClelland M, Escalante-Semerena JC, Lee MD.

Campylobacter jejuni uses energy taxis and a dehydrogenase enzyme for l-fucose chemotaxis.

Zhou B, Garber JM, Vlach J, Azadi P, Ng KKS, Escalante-Semerena JC, Szymanski CM.

In Salmonella enterica, the pathogenicity island 2 (SPI-2) regulator PagR regulates its own expression and the expression of a five-gene operon that encodes transketolase C

Parks, AR, McCormick, RD, Byrne, JA, Escalante-Semerena, JC.

The coenzyme B12 precursor 5,6-dimethylbenzimidazole is a flavin antagonist in Salmonella

Malalasekara, LR, and Escalante-Semerena, JC.

Structural studies of the phosphoribosyltransferase involved in cobamide biosynthesis in methanogenic archaea and cyanobacteria

Jeter, VL, AH Schwarzwalder, I Rayment, I, and JC Escalante-Semerena..

Acinetobacter baumannii catabolizes ethanolamine in the absence of a metabolosome and converts cobinamide into adenosylated cobamides

Villa, LA, and JC Escalanate-Semerena..

Localization and interaction studies of the Salmonella enterica ethanolamine ammonia-lyase (EutBC), its reactivase (EutA) and the EutT corrinoid adenosyltransferase

Costa, FG, and JC Escalante-Semerena..

A method for the efficient adenosylation of corrinoids

Costa, FG, EA Villa, and JC Escalante-Semerena..

A method for the production, purification and liposome reconstitution of cobamide synthase

Villa, EA, and JC Escalante-Semerena..

A method for the isolation of ?-ribazole from vitamin B12, and its enzymatic conversion to ?-ribazole 5'-phosphate

Malalasekara, L., and JC Escalante-Semerena.

Elevated levels of an enzyme involved in coenzyme B12 biosynthesis kills Escherichia coli

Jeter, VL and JC Escalante-Semerena..

Sirtuin-dependent reversible lysine acetylation controls the activity of acetyl-Coenzyme A synthetase in Campylobacter jejuni

Jeter, VL, and JC Escalante-Semerena..

Functional studies of ?-riboside activation by the ?-ribazole kinase (CblS) from Geobacillus kaustophilus

Mattes, TA, L. Malalasekara, and JC Escalante-Semerena..

Insights into the relationship between cobamide synthase and the cell membrane

Jeter, VL, and JC Escalante-Semerena..

Modulation of the bacterial CobB sirtuin deacylase activity by N-terminal acetylation.

Parks, AR, and JC Escalante-Semerena..

Small-Molecule Acetylation by GCN5-RelatedN-Acetyltransferases in Bacteria.

Burckhardt, RM, and JC Escalante-Semerena..

Mutational and functional analyses of substrate binding and catalysis of the Listeria monocytogenes EutT ATP:Co(I)rrinoid Adenosyltransferase.

Costa, FG, ED Greenhalgh, TC Brunold, and JC Escalante-Semerena..

New AMP-forming acid:CoA ligases from Streptomyces lividans, some of which are posttranslationally regulated by reversible lysine acetylation.

Burckhardt, RM, CM VanDrisse, AC Tucker, JC Escalante-Semerena. .

Protein Acetylation in Bacteria.

VanDrisse, CM, and JC Escalante-Semerena..

The The L-Thr kinase/L-Thr-phosphate decarboxylase (CobD) enzyme from Methanosarcina mazei G�1 contains metallocenters needed for optimal activity

Tavares, NK, N. Stracey, TC Brunold, JC Escalante-Semerena..

Staphylococcus aureus modulates the activity of acetyl-Coenzyme A synthetase (Acs) by sirtuin-dependent reversible lysine acetylation

Burckhardt, RM, BA Buckner, JC Escalante-Semerena..

Insights into the function of the N-acetyltransferase SatA that detoxifies streptothricin in Bacillus subtilis and Bacillus anthracis

Burckhardt, RM, JC Escalante-Semerena..

A new class of phosphoribosyltransferase involved in cobamide biosynthesis is found in methanogenic archaea and cyanobacteria

Jeter, VL, TA Mattes, NR Beattie, JC Escalante-Semerena..

In Salmonella enterica, OatA (formerly YjgM) uses O-acetyl-Serine and Acetyl-CoA to synthesize N,O-diacetylserine, which upregulates cysteine biosynthesis

VanDrisse, CM, and JC Escalante-Semerena..

Small-molecule acetylation controls the degradation of benzoate and photosynthesis in Rhodopseudomonas palustris

VanDrisse, CM, JC Escalante-Semerena..

Rhodobacterales use a unique L-threonine kinase for the assembly of the nucleotide loop of coenzyme B12

Tavares, NK, CM VanDrisse, JC Escalante-Semerena..

A new class of EutT ATP:Co(I)rrinoid adenosyltransferases found in Listeria monocytogenes and other Firmicutes does not require a metal ion for activity

Costa, FG, JC Escalante-Semerena..

Spectroscopic study of the EutT adenosyltransferase from Listeria monocytogenes: Evidence for the formation of a four-coordinate cob(II)alamin intermediate

Stracey, N., FG Costa, JC Escalante-Semerena, TC. Brunold..

Facile isolation of alpha-ribazole from vitamin B12 hydrolysates using boronate affinity chromatography

Mattes, TA, and JC Escalante-Semerena..

The Methanosarcina mazei MM2060 gene encodes a bifunctional kinase/decarboxylase enzyme involved in cobamide biosynthesis

Tavares, NK, CL Zayas, and JC Escalante-Semerena. .

In Streptomyces lividans, acetyl-CoA synthetase activity is controlled by O-serine and N(epsilon) acetylation

VanDrisse, CM, JC Escalante-Semerena..

The PrpF protein of Shewanella oneidensis MR-1 catalyzes the isomerization of 2-methyl-cis-aconitate during the catabolism of propionate via the AcnD-dependent 2-methylcitric acid cycle

Rocco, CJ, KM Wetterhorn, GS Garvey, I Rayment, JC Escalante-Semerena..

Cobalamin Biosynthesis and Insertion

Mattes, TA, E Deery, MJ Warren, JC Escalante-Semerena..

In Bacillus subtilis, the SatA (formerly YyaR) acetyltransferase detoxifies streptothricin via lysine acetylation

Burckhardt, RM, JC Escalante-Semerena..

A toxin involved in Salmonella persistence regulates its activity by acetylating its cognate antitoxin, a modification reversed by CobB sirtuin deacetylase

VanDrisse CM, AR Parks, JC Escalante-Semerena..

Spectroscopic studies of the EutT adenosyltransferase from Salmonella enterica: Evidence of a tetrathydrally coordinated transition metal cofactor with cysteine ligation

Pallares, IG, TC Moore, JC Escalante-Semerena, TC Brunold..

Salmonella enterica synthesizes 5,6-dimethylbenzimidazolyl-(DMB)-?-riboside. Why some Firmicutes do not require the canonical DMB activation system to synthesize adenosylcobalamin

Mattes, TA, JC Escalante-Semerena..

Identification of phosphinothricin acetyltransferases using in vivo, in vitro and bioinformatics analyses.

VanDrisse, CM, KL Hentchel, JC Escalante-Semerena..

A snapshot of evolution in action: emergence of new heme transport function derived from a coenzyme B12 biosynthetic enzyme

Tavares, NK, JC Escalante-Semerena..

Resonance Raman spectroscopic study of the interaction between Co(II)rrinoids and the ATP:Corrinoid adenosyltransferase PduO from Lactobacillus reuteri

Park, K, PE Mera, JC Escalante-Semerena, TC Brunold..

New high-cloning-efficiency vectors for complementation studies and recombinant protein overproduction in Escherichia coli and Salmonella enterica

VanDrisse, CM, JC Escalante-Semerena..

Spectroscopic studies of the EutT adenosyltransferase from Salmonella enterica: Mechanism of four coordinate Co(II)Cbl formation

Pallares, IG, TC Moore, JC Escalante-Semerena, TC Brunold..

Complex regulation of the sirtuin-dependent reversible lysine acetylation system of Salmonella enterica

Hentchel, KL., and JC Escalante-Semerena..

Solution structural studies of GTP:adenosylcobinamide-phosphateguanylyltransferase (CobY) from Methanocaldococcus jannaschii

Singarapu, KS, M Otte, M Tonelli, WM Westler, JC Escalante-Semerena, JL Markley.

The EutQ and EutP proteins are novel acetate kinases involved in ethanolamine catabolism: Physiological implications for the function of the ethanolamine metabolosome in Salmonella enterica

Moore, TC, and JC Escalante-Semerena. .

Acylation of biomolecules in prokaryotes: a widespread strategy for the control of biological function and metabolic stress

Hentchel, K. L., and J. C. Escalante-Semerena..

Deciphering the regulatory circuitry that controls reversible lysine acetylation in Salmonella enterica

Hentchel, K.L., S. Thao, P. J. Intile, and J. C. Escalante-Semerena..

Phylogenetic and amino acid conservation analyses of bacterial L-aspartate-?-decarboxylase and of its zymogen-maturation protein reveal a putative interaction domain

Stuecker, TN, S Bramhacharya, KM Hodge-Hanson, G.Suen, JC Escalante-Semerena.

Unprecedented mechanism employed by the Salmonella enterica EutT ATP:Co(I)rrinoid adenosyltransferase precludes adenosylation of incomplete Co(II)rrinoids

Park, K., P. E. Mera, T. C. Moore, J. C. Escalante-Semerena, and T. C. Brunold. .

The structure of S. lividans acetoacetyl-CoA synthetase shows a novel interaction between the C-terminal extension and the N-terminal domain

Mitchell, C. A., A. C. Tucker, J. C. Escalante-Semerena, and A. M. Gulick.

In Salmonella enterica, the Gcn5-related acetyltransferase MddA (formerly YncA) acetylates methionine sulfoximine and methionine sulfone, blocking their toxic effects

Hentchel, K. L., and J. C. Escalante-Semerena.

Insights into the specificity of lysine acetyltransferases

Tucker, A. C., K. A. Taylor, K. C. Rank, I. Rayment, and J. C. Escalante-Semerena.

Sprectoscopic studies of the Salmonella enterica adenosyltransferase enzyme SeCobA: Molecular insight into the mechanism of substrate cob(II)alamin activation

Pallares, IG, TC Moore, JC Escalante-Semerena, TC Brunold.

Acetyl-CoA synthetase is acetylated on multiple lysine residues by a protein acetyltransferase with single GNAT domain in Saccharopolyspora erythraea

You, D, L-L Yao, D Huang, JC Escalante-Semerena, B-C Ye.

Determinants within the C-terminal domain of Streptomyces lividans acetyl-CoA synthetase that block acetylation of its active site lysine in vitro by the protein acetyltransferase (Pat) enzyme

Tucker, AC, & JC Escalante-Semerena.

The EutT enzyme of Salmonella enterica is a unique ATP:Cob(I)alamin adenosyltransferase metalloprotein that requires ferrous ions for maximal activity

Moore, T C, PE Mera, & JC Escalante-Semerena.

The acetylation motif in AMP-forming acyl-CoA synthetases contains residues critical for acetylation and recognition by the protein acetyltransferase Pat of Rhodopseudomonas palustris

Crosby, HA & JC Escalante-Semerena.

Dissecting cobamide diversity through structural and functional analyses of the base-activating CobT enzyme of Salmonella enterica

Chan, CH.

Bacillus megaterium has both a functional BluB protein required for DMB synthesis and a related flavoprotein that forms a stable radical species

Collins, H. et al.

Acetoacetyl-CoA synthetase activity is controlled by a protein acetyltransferase with unique domain organization in Streptomyces lividans

Tucker, AC & Escalante-Semerena, JC.

Structural Insights into the Substrate Specificity of the Rhodopseudomonas palustris Protein Acetyltransferase RpPat. Identification of a loop critical for recognition by RpPat

Crosby, HA et al.

Structural insights into the mechanism of four-coordinate cob(II)alamin formation in the active site of the Salmonella enterica ATP:co(I)rrinoid adenosyltransferase (CobA) enzyme: Critical role of residues Phe91 and Trp93

Moore, TC et al.

Structure-Guided Expansion of the Substrate Range of Methylmalonyl Coenzyme A Synthetase (MatB) of Rhodopseudomonas palustris.

Crosby HA, Rank KC, Rayment I, Escalante-Semerena JC.

System-wide studies of N-lysine acetylation in Rhodopseudomonas palustris reveal substrate specificity of protein acetyltransferases.

Crosby HA, Pelletier DA, Hurst GB, Escalante-Semerena JC.

The missing link in coenzyme A biosynthesis: PanM (formerly YhhK), a yeast GCN5 acetyltransferase homologue triggers aspartate decarboxylase (PanD) maturation in Salmonella enterica.

Stuecker TN, Hodge KM, Escalante-Semerena JC.

PanM, an acetyl-coenzyme A sensor required for maturation of L-aspartate decarboxylase (PanD).

Stuecker TN, Tucker AC, Escalante-Semerena JC.

Spectroscopic characterization of active-site variants of the PduO-type ATP:corrinoid adenosyltransferase from Lactobacillus reuteri: insights into the mechanism of four-coordinate Co(II)corrinoid formation.

Park K, Mera PE, Escalante-Semerena JC, Brunold TC.

A positive selection approach identifies residues important for folding of Salmonella enterica Pat, an N(?)-lysine acetyltransferase that regulates central metabolism enzymes.

Thao S, Escalante-Semerena JC.

Structural insights into the function of the nicotinate mononucleotide:phenol/p-cresol phosphoribosyltransferase (ArsAB) enzyme from Sporomusa ovata.

Newmister SA, Chan CH, Escalante-Semerena JC, Rayment I.

Structure and mutational analysis of the archaeal GTP:AdoCbi-P guanylyltransferase (CobY) from Methanocaldococcus jannaschii: insights into GTP binding and dimerization.

Newmister SA, Otte MM, Escalante-Semerena JC, Rayment I.

In Salmonella enterica, the sirtuin-dependent protein acylation/deacylation system (SDPADS) maintains energy homeostasis during growth on low concentrations of acetate.

Chan CH, Garrity J, Crosby HA, Escalante-Semerena JC.

Control of protein function by reversible N?-lysine acetylation in bacteria.

Thao S, Escalante-Semerena JC.

Biochemical and thermodynamic analyses of Salmonella enterica Pat, a multidomain, multimeric N(?)-lysine acetyltransferase involved in carbon and energy metabolism.

Thao S, Escalante-Semerena JC.

ArsAB, a novel enzyme from Sporomusa ovata activates phenolic bases for adenosylcobamide biosynthesis.

Chan CH, Escalante-Semerena JC.

Structure of Sir2Tm bound to a propionylated peptide.

Bheda P, Wang JT, Escalante-Semerena JC, Wolberger C.

Functional analysis of the nicotinate mononucleotide:5,6-dimethylbenzimidazole phosphoribosyltransferase (CobT) enzyme, involved in the late steps of coenzyme B12 biosynthesis in Salmonella enterica.

Claas KR, Parrish JR, Maggio-Hall LA, Escalante-Semerena JC.

A new pathway for the synthesis of ?-ribazole-phosphate in Listeria innocua.

Gray MJ, Escalante-Semerena JC.

Biologically active isoforms of CobB sirtuin deacetylase in Salmonella enterica and Erwinia amylovora.

Tucker AC, Escalante-Semerena JC.

Reversible N epsilon-lysine acetylation regulates the activity of acyl-CoA synthetases involved in anaerobic benzoate catabolism in Rhodopseudomonas palustris.

Crosby HA, Heiniger EK, Harwood CS, Escalante-Semerena JC.

Dihydroflavin-driven adenosylation of 4-coordinate Co(II) corrinoids: are cobalamin reductases enzymes or electron transfer proteins?

Mera PE, Escalante-Semerena JC.

Multiple roles of ATP:cob(I)alamin adenosyltransferases in the conversion of B12 to coenzyme B12.

Mera PE, Escalante-Semerena JC.

In Salmonella enterica, 2-methylcitrate blocks gluconeogenesis.

Rocco CJ, Escalante-Semerena JC.

N?-lysine acetylation of a bacterial transcription factor inhibits Its DNA-binding activity.

Thao S, Chen CS, Zhu H, Escalante-Semerena JC.

Residue Phe112 of the human-type corrinoid adenosyltransferase (PduO) enzyme of Lactobacillus reuteri is critical to the formation of the four-coordinate Co(II) corrinoid substrate and to the activity of the enzyme.

Mera PE, St Maurice M, Rayment I, Escalante-Semerena JC.

Biochemical characterization of the GTP:adenosylcobinamide-phosphate guanylyltransferase (CobY) enzyme of the hyperthermophilic archaeon Methanocaldococcus jannaschii.

Otte MM, Escalante-Semerena JC.

The cobinamide amidohydrolase (cobyric acid-forming) CbiZ enzyme: a critical activity of the cobamide remodelling system of Rhodobacter sphaeroides.

Gray MJ, Escalante-Semerena JC.

In vivo analysis of cobinamide salvaging in Rhodobacter sphaeroides strain 2.4.1.

Gray MJ, Escalante-Semerena JC.

Involvement of the Cra global regulatory protein in the expression of the iscRSUA operon, revealed during studies of tricarballylate catabolism in Salmonella enterica.

Lewis JA, Boyd JM, Downs DM, Escalante-Semerena JC.

Regulation of expression of the tricarballylate utilization operon (tcuABC) of Salmonella enterica.

Lewis JA, Stamper LW, Escalante-Semerena JC.

In Bacillus subtilis, the sirtuin protein deacetylase, encoded by the srtN gene (formerly yhdZ), and functions encoded by the acuABC genes control the activity of acetyl coenzyme A synthetase.

Gardner JG, Escalante-Semerena JC.

Structural characterization of a human-type corrinoid adenosyltransferase confirms that coenzyme B12 is synthesized through a four-coordinate intermediate.

St Maurice M, Mera P, Park K, Brunold TC, Escalante-Semerena JC, Rayment I.

Construction and use of new cloning vectors for the rapid isolation of recombinant proteins from Escherichia coli.

Rocco CJ, Dennison KL, Klenchin VA, Rayment I, Escalante-Semerena JC.

Kinetic and spectroscopic studies of the ATP:corrinoid adenosyltransferase PduO from Lactobacillus reuteri: substrate specificity and insights into the mechanism of Co(II)corrinoid reduction.

Park K, Mera PE, Escalante-Semerena JC, Brunold TC.

The genome of Rhodobacter sphaeroides strain 2.4.1 encodes functional cobinamide salvaging systems of archaeal and bacterial origins.

Gray MJ, Tavares NK, Escalante-Semerena JC.

Syntheses and characterization of vitamin B12-Pt(II) conjugates and their adenosylation in an enzymatic assay.

Ruiz-S�nchez P, Mundwiler S, Spingler B, Buan NR, Escalante-Semerena JC, Alberto R.

Biochemical and mutational analyses of AcuA, the acetyltransferase enzyme that controls the activity of the acetyl coenzyme a synthetase (AcsA) in Bacillus subtilis.

Gardner JG, Escalante-Semerena JC.

Salmonella enterica requires ApbC function for growth on tricarballylate: evidence of functional redundancy between ApbC and IscU.

Boyd JM, Lewis JA, Escalante-Semerena JC, Downs DM.

Structural and functional analyses of the human-type corrinoid adenosyltransferase (PduO) from Lactobacillus reuteri.

Mera PE, St Maurice M, Rayment I, Escalante-Semerena JC.

The three-dimensional crystal structure of the PrpF protein of Shewanella oneidensis complexed with trans-aconitate: insights into its biological function.

Garvey GS, Rocco CJ, Escalante-Semerena JC, Rayment I.

Structural characterization of the active site of the PduO-type ATP:Co(I)rrinoid adenosyltransferase from Lactobacillus reuteri.

St Maurice M, Mera PE, Taranto MP, Sesma F, Escalante-Semerena JC, Rayment I.

Conversion of cobinamide into adenosylcobamide in bacteria and archaea.

Escalante-Semerena JC.

Tricarballylate catabolism in Salmonella enterica. The TcuB protein uses 4Fe-4S clusters and heme to transfer electrons from FADH2 in the tricarballylate dehydrogenase (TcuA) enzyme to electron acceptors in the cell membrane.

Lewis JA, Escalante-Semerena JC.

In vivo and in vitro analyses of single-amino acid variants of the Salmonella enterica phosphotransacetylase enzyme provide insights into the function of its N-terminal domain.

Brinsmade SR, Escalante-Semerena JC.

Reassessment of the late steps of coenzyme B12 synthesis in Salmonella enterica: evidence that dephosphorylation of adenosylcobalamin-5'-phosphate by the CobC phosphatase is the last step of the pathway.

Zayas CL, Escalante-Semerena JC.

Single-enzyme conversion of FMNH2 to 5,6-dimethylbenzimidazole, the lower ligand of B12.

Gray MJ, Escalante-Semerena JC.

The CbiB protein of Salmonella enterica is an integral membrane protein involved in the last step of the de novo corrin ring biosynthetic pathway.

Zayas CL, Claas K, Escalante-Semerena JC.

N-lysine propionylation controls the activity of propionyl-CoA synthetase.

Garrity J, Gardner JG, Hawse W, Wolberger C, Escalante-Semerena JC.

The thiamine kinase (YcfN) enzyme plays a minor but significant role in cobinamide salvaging in Salmonella enterica.

Otte MM, Woodson JD, Escalante-Semerena JC.

Control of acetyl-coenzyme A synthetase (AcsA) activity by acetylation/deacetylation without NAD(+) involvement in Bacillus subtilis.

Gardner JG, Grundy FJ, Henkin TM, Escalante-Semerena JC.

Purification and initial biochemical characterization of ATP:Cob(I)alamin adenosyltransferase (EutT) enzyme of Salmonella enterica.

Buan NR, Escalante-Semerena JC.

The cobZ gene of Methanosarcina mazei Go1 encodes the nonorthologous replacement of the alpha-ribazole-5'-phosphate phosphatase (CobC) enzyme of Salmonella enterica.

Zayas CL, Woodson JD, Escalante-Semerena JC.

Studies of the CobA-type ATP:Co(I)rrinoid adenosyltransferase enzyme of Methanosarcina mazei strain Go1.

Buan NR, Rehfeld K, Escalante-Semerena JC.

The cbiS gene of the archaeon Methanopyrus kandleri AV19 encodes a bifunctional enzyme with adenosylcobinamide amidohydrolase and alpha-ribazole-phosphate phosphatase activities.

Woodson JD, Escalante-Semerena JC.

The FAD-dependent tricarballylate dehydrogenase (TcuA) enzyme of Salmonella enterica converts tricarballylate into cis-aconitate.

Lewis JA, Escalante-Semerena JC.

Computer-assisted docking of flavodoxin with the ATP:Co(I)rrinoid adenosyltransferase (CobA) enzyme reveals residues critical for protein-protein interactions but not for catalysis.

Buan NR, Escalante-Semerena JC.

Residue Leu-641 of Acetyl-CoA synthetase is critical for the acetylation of residue Lys-609 by the Protein acetyltransferase enzyme of Salmonella enterica.

Starai VJ, Gardner JG, Escalante-Semerena JC.

Minimal functions and physiological conditions required for growth of salmonella enterica on ethanolamine in the absence of the metabolosome.

Brinsmade SR, Paldon T, Escalante-Semerena JC.

Spectroscopic and computational studies of the ATP:corrinoid adenosyltransferase (CobA) from Salmonella enterica: insights into the mechanism of adenosylcobalamin biosynthesis.

Stich TA, Buan NR, Escalante-Semerena JC, Brunold TC.

Acetate excretion during growth of Salmonella enterica on ethanolamine requires phosphotransacetylase (EutD) activity, and acetate recapture requires acetyl-CoA synthetase (Acs) and phosphotransacetylase (Pta) activities.

Starai VJ, Garrity J, Escalante-Semerena JC.

ABC transporter for corrinoids in Halobacterium sp. strain NRC-1.

Woodson JD, Reynolds AA, Escalante-Semerena JC.

CbiZ, an amidohydrolase enzyme required for salvaging the coenzyme B12 precursor cobinamide in archaea.

Woodson JD, Escalante-Semerena JC.

Acetyl-coenzyme A synthetase (AMP forming).

Starai VJ, Escalante-Semerena JC.

The acnD genes of Shewenella oneidensis and Vibrio cholerae encode a new Fe/S-dependent 2-methylcitrate dehydratase enzyme that requires prpF function in vivo.

Grimek TL, Escalante-Semerena JC.

2-Methylcitrate-dependent activation of the propionate catabolic operon (prpBCDE) of Salmonella enterica by the PrpR protein.

Palacios S, Escalante-Semerena JC.

The Tricarballylate utilization (tcuRABC) genes of Salmonella enterica serovar Typhimurium LT2.

Lewis JA, Horswill AR, Schwem BE, Escalante-Semerena JC.

The eutD gene of Salmonella enterica encodes a protein with phosphotransacetylase enzyme activity.

Brinsmade SR, Escalante-Semerena JC.

A link between transcription and intermediary metabolism: a role for Sir2 in the control of acetyl-coenzyme A synthetase.

Starai VJ, Takahashi H, Boeke JD, Escalante-Semerena JC.

Identification of the protein acetyltransferase (Pat) enzyme that acetylates acetyl-CoA synthetase in Salmonella enterica.

Starai VJ, Escalante-Semerena JC.

The last step in coenzyme B(12) synthesis is localized to the cell membrane in bacteria and archaea.

Maggio-Hall LA, Claas KR, Escalante-Semerena JC.

The eutT gene of Salmonella enterica Encodes an oxygen-labile, metal-containing ATP:corrinoid adenosyltransferase enzyme.

Buan NR, Suh SJ, Escalante-Semerena JC.

Residues C123 and D58 of the 2-methylisocitrate lyase (PrpB) enzyme of Salmonella enterica are essential for catalysis.

Grimek TL, Holden H, Rayment I, Escalante-Semerena JC.

Alpha-5,6-dimethylbenzimidazole adenine dinucleotide (alpha-DAD), a putative new intermediate of coenzyme B12 biosynthesis in Salmonella typhimurium.

Maggio-Hall LA, Escalante-Semerena JC.

The cobY gene of the archaeon Halobacterium sp. strain NRC-1 is required for de novo cobamide synthesis.

Woodson JD, Peck RF, Krebs MP, Escalante-Semerena JC.

Formation of the dimethylbenzimidazole ligand of coenzyme B(12) under physiological conditions by a facile oxidative cascade.

Maggio-Hall LA, Dorrestein PC, Escalante-Semerena JC, Begley TP.

The 1.75 A crystal structure of acetyl-CoA synthetase bound to adenosine-5'-propylphosphate and coenzyme A.

Gulick AM, Starai VJ, Horswill AR, Homick KM, Escalante-Semerena JC.

A new pathway for salvaging the coenzyme B12 precursor cobinamide in archaea requires cobinamide-phosphate synthase (CbiB) enzyme activity.

Woodson JD, Zayas CL, Escalante-Semerena JC.

Short-chain fatty acid activation by acyl-coenzyme A synthetases requires SIR2 protein function in Salmonella enterica and Saccharomyces cerevisiae.

Starai VJ, Takahashi H, Boeke JD, Escalante-Semerena JC.

Propionyl coenzyme A is a common intermediate in the 1,2-propanediol and propionate catabolic pathways needed for expression of the prpBCDE operon during growth of Salmonella enterica on 1,2-propanediol.

Palacios S, Starai VJ, Escalante-Semerena JC.

Capture of a labile substrate by expulsion of water molecules from the active site of nicotinate mononucleotide:5,6-dimethylbenzimidazole phosphoribosyltransferase (CobT) from Salmonella enterica.

Cheong CG, Escalante-Semerena JC, Rayment I.

Three-dimensional structure of the L-threonine-O-3-phosphate decarboxylase (CobD) enzyme from Salmonella enterica.

Cheong CG, Bauer CB, Brushaber KR, Escalante-Semerena JC, Rayment I.

Structural studies of the L-threonine-O-3-phosphate decarboxylase (CobD) enzyme from Salmonella enterica: the apo, substrate, and product-aldimine complexes.

Cheong CG, Escalante-Semerena JC, Rayment I.

The ATP:Co(I)rrinoid adenosyltransferase (CobA) enzyme of Salmonella enterica requires the 2'-OH group of ATP for function and yields inorganic triphosphate as its reaction byproduct.

Fonseca MV, Buan NR, Horswill AR, Rayment I, Escalante-Semerena JC.

Pentaerythritol propoxylate: a new crystallization agent and cryoprotectant induces crystal growth of 2-methylcitrate dehydratase.

Gulick AM, Horswill AR, Thoden JB, Escalante-Semerena JC, Rayment I.

Characterization of the propionyl-CoA synthetase (PrpE) enzyme of Salmonella enterica: residue Lys592 is required for propionyl-AMP synthesis.

Horswill AR, Escalante-Semerena JC.

Sir2-dependent activation of acetyl-CoA synthetase by deacetylation of active lysine.

Starai VJ, Celic I, Cole RN, Boeke JD, Escalante-Semerena JC.

The biosynthesis of adenosylcobalamin (vitamin B12).

Warren MJ, Raux E, Schubert HL, Escalante-Semerena JC.

Three-dimensional structure of ATP:corrinoid adenosyltransferase from Salmonella typhimurium in its free state, complexed with MgATP, or complexed with hydroxycobalamin and MgATP.

Bauer CB, Fonseca MV, Holden HM, Thoden JB, Thompson TB, Escalante-Semerena JC, Rayment I.

Structural investigation of the biosynthesis of alternative lower ligands for cobamides by nicotinate mononucleotide: 5,6-dimethylbenzimidazole phosphoribosyltransferase from Salmonella enterica.

Cheong CG, Escalante-Semerena JC, Rayment I.

In vitro conversion of propionate to pyruvate by Salmonella enterica enzymes: 2-methylcitrate dehydratase (PrpD) and aconitase Enzymes catalyze the conversion of 2-methylcitrate to 2-methylisocitrate.

Horswill AR, Escalante-Semerena JC.

The coenzyme b12 analog 5'-deoxyadenosylcobinamide-gdp supports catalysis by methylmalonyl-coa mutase in the absence of trans-ligand coordination.

Chowdhury S, Thomas MG, Escalante-Semerena JC, Banerjee R.

Studies of propionate toxicity in Salmonella enterica identify 2-methylcitrate as a potent inhibitor of cell growth.

Horswill AR, Dudding AR, Escalante-Semerena JC.

An in vitro reducing system for the enzymic conversion of cobalamin to adenosylcobalamin.

Fonseca MV, Escalante-Semerena JC.

Analysis of the adenosylcobinamide kinase/adenosylcobinamide-phosphate guanylyltransferase (CobU) enzyme of Salmonella typhimurium LT2. Identification of residue His-46 as the site of guanylylation.

Thomas MG, Thompson TB, Rayment I, Escalante-Semerena JC.

Reduction of Cob(III)alamin to Cob(II)alamin in Salmonella enterica serovar typhimurium LT2.

Fonseca MV, Escalante-Semerena JC.

Identification of an alternative nucleoside triphosphate: 5'-deoxyadenosylcobinamide phosphate nucleotidyltransferase in Methanobacterium thermoautotrophicum delta H.

Thomas MG, Escalante-Semerena JC.

A phylogenetically conserved NAD+-dependent protein deacetylase activity in the Sir2 protein family.

Smith JS, Brachmann CB, Celic I, Kenna MA, Muhammad S, Starai VJ, Avalos JL, Escalante-Semerena JC, Grubmeyer C, Wolberger C, Boeke JD.

prpR, ntrA, and ihf functions are required for expression of the prpBCDE operon, encoding enzymes that catabolize propionate in Salmonella enterica serovar typhimurium LT2.

Palacios S, Escalante-Semerena JC.

Impact of genomics and genetics on the elucidation of bacterial metabolism.

Downs DM, Escalante-Semerena JC.

The three-dimensional structures of nicotinate mononucleotide:5,6- dimethylbenzimidazole phosphoribosyltransferase (CobT) from Salmonella typhimurium complexed with 5,6-dimethybenzimidazole and its reaction products determined to 1.9 A resolution.

Cheong CG, Escalante-Semerena JC, Rayment I.

Three-dimensional structure of adenosylcobinamide kinase/adenosylcobinamide phosphate guanylyltransferase (CobU) complexed with GMP: evidence for a substrate-induced transferase active site.

Thompson TB, Thomas MG, Escalante-Semerena JC, Rayment I.

In vitro synthesis of the nucleotide loop of cobalamin by Salmonella typhimurium enzymes.

Maggio-Hall LA, Escalante-Semerena JC.

The prpE gene of Salmonella typhimurium LT2 encodes propionyl-CoA synthetase.

Horswill AR, Escalante-Semerena JC.

Salmonella typhimurium LT2 catabolizes propionate via the 2-methylcitric acid cycle.

Horswill AR, Escalante-Semerena JC.

Molecular characterization of eutF mutants of Salmonella typhimurium LT2 identifies eutF lesions as partial-loss-of-function tonB alleles.

Thomas MG, O'Toole GA, Escalante-Semerena JC.

Three-dimensional structure of adenosylcobinamide kinase/adenosylcobinamide phosphate guanylyltransferase from Salmonella typhimurium determined to 2.3 A resolution,.

Thompson TB, Thomas MG, Escalante-Semerena JC, Rayment I.

CobB, a new member of the SIR2 family of eucaryotic regulatory proteins, is required to compensate for the lack of nicotinate mononucleotide:5,6-dimethylbenzimidazole phosphoribosyltransferase activity in cobT mutants during cobalamin biosynthesis in Salmonella typhimurium LT2.

Tsang AW, Escalante-Semerena JC.

Studies of regulation of expression of the propionate (prpBCDE) operon provide insights into how Salmonella typhimurium LT2 integrates its 1,2-propanediol and propionate catabolic pathways.

Tsang AW, Horswill AR, Escalante-Semerena JC.

CobD, a novel enzyme with L-threonine-O-3-phosphate decarboxylase activity, is responsible for the synthesis of (R)-1-amino-2-propanol O-2-phosphate, a proposed new intermediate in cobalamin biosynthesis in Salmonella typhimurium LT2.

Brushaber KR, O'Toole GA, Escalante-Semerena JC.

High levels of transcription factor RpoS (sigma S) in mviA mutants negatively affect 1,2-propanediol-dependent transcription of the cob/pdu regulon of Salmonella typhimurium LT2.

Rondon MR, Escalante-Semerena JC.

Purification and characterization of CobT, the nicotinate-mononucleotide:5,6-dimethylbenzimidazole phosphoribosyltransferase enzyme from Salmonella typhimurium LT2.

Trzebiatowski JR, Escalante-Semerena JC.

Biochemistry and molecular genetics of cobalamin biosynthesis.

Rondon MR, Trzebiatowski JR, Escalante-Semerena JC.

Integration host factor is required for 1,2-propanediol-dependent transcription of the cob/pdu regulon in Salmonella typhimurium LT2.

Rondon MR, Escalante-Semerena JC.

Propionate catabolism in Salmonella typhimurium LT2: two divergently transcribed units comprise the prp locus at 8.5 centisomes, prpR encodes a member of the sigma-54 family of activators, and the prpBCDE genes constitute an operon.

Horswill AR, Escalante-Semerena JC.

cobB function is required for catabolism of propionate in Salmonella typhimurium LT2: evidence for existence of a substitute function for CobB within the 1,2-propanediol utilization (pdu) operon.

Tsang AW, Escalante-Semerena JC.

In vitro analysis of the interactions between the PocR regulatory protein and the promoter region of the cobalamin biosynthetic (cob) operon of Salmonella typhimurium LT2.

Rondon MR, Escalante-Semerena JC.

Identification of a new prp locus required for propionate catabolism in Salmonella typhimurium LT2.

Hammelman TA, O'Toole GA, Trzebiatowski JR, Tsang AW, Rank D, Escalante-Semerena JC.

Purification and characterization of the bifunctional CobU enzyme of Salmonella typhimurium LT2. Evidence for a CobU-GMP intermediate.

O'Toole GA, Escalante-Semerena JC.

DNA polymerase I function is required for the utilization of ethanolamine, 1,2-propanediol, and propionate by Salmonella typhimurium LT2.

Rondon MR, Horswill AR, Escalante-Semerena JC.

Purification and initial characterization of the ATP:corrinoid adenosyltransferase encoded by the cobA gene of Salmonella typhimurium.

Suh S, Escalante-Semerena JC.

Glutathione is required for maximal transcription of the cobalamin biosynthetic and 1,2-propanediol utilization (cob/pdu) regulon and for the catabolism of ethanolamine, 1,2-propanediol, and propionate in Salmonella typhimurium LT2.

Rondon MR, Kazmierczak R, Escalante-Semerena JC.

The cobT gene of Salmonella typhimurium encodes the NaMN: 5,6-dimethylbenzimidazole phosphoribosyltransferase responsible for the synthesis of N1-(5-phospho-alpha-D-ribosyl)-5,6-dimethylbenzimidazole, an intermediate in the synthesis of the nucleotide loop of cobalamin.

Trzebiatowski JR, O'Toole GA, Escalante-Semerena JC.

The cobC gene of Salmonella typhimurium codes for a novel phosphatase involved in the assembly of the nucleotide loop of cobalamin.

O'Toole GA, Trzebiatowski JR, Escalante-Semerena JC.

cobU-dependent assimilation of nonadenosylated cobinamide in cobA mutants of Salmonella typhimurium.

O'Toole GA, Escalante-Semerena JC.

Analysis of mutants of Salmonella typhimurium defective in the synthesis of the nucleotide loop of cobalamin.

O'Toole GA, Rondon MR, Escalante-Semerena JC.

Cloning, sequencing and overexpression of cobA which encodes ATP:corrinoid adenosyltransferase in Salmonella typhimurium.

Suh SJ, Escalante-Semerena JC.

Identification of 5,6-dimethylbenzimidazole as the Co alpha ligand of the cobamide synthesized by Salmonella typhimurium. Nutritional characterization of mutants defective in biosynthesis of the imidazole ring.

Johnson MG, Escalante-Semerena JC.

The CobII and CobIII regions of the cobalamin (vitamin B12) biosynthetic operon of Salmonella typhimurium.

Escalante-Semerena JC, Johnson MG, Roth JR.

The poc locus is required for 1,2-propanediol-dependent transcription of the cobalamin biosynthetic (cob) and propanediol utilization (pdu) genes of Salmonella typhimurium.

Rondon MR, Escalante-Semerena JC.

Identification and initial characterization of the eutF locus of Salmonella typhimurium.

O'Toole GA, Escalante-Semerena JC.

cobA function is required for both de novo cobalamin biosynthesis and assimilation of exogenous corrinoids in Salmonella typhimurium.

Escalante-Semerena JC, Suh SJ, Roth JR.

A simplified methylcoenzyme M methylreductase assay with artificial electron donors and different preparations of component C from Methanobacterium thermoautotrophicum delta H.

Hartzell PL, Escalante-Semerena JC, Bobik TA, Wolfe RS.

Regulation of cobalamin biosynthetic operons in Salmonella typhimurium.

Escalante-Semerena JC, Roth JR.

Coenzyme F420 dependence of the methylenetetrahydromethanopterin dehydrogenase of Methanobacterium thermoautotrophicum.

Hartzell PL, Zvilius G, Escalante-Semerena JC, Donnelly MI.

Tetrahydromethanopterin-dependent methanogenesis from non-physiological C1 donors in Methanobacterium thermoautotrophicum.

Escalante-Semerena JC, Wolfe RS.

Methenyl-tetrahydromethanopterin cyclohydrolase in cell extracts of Methanobacterium.

Donnelly MI, Escalante-Semerena JC, Rinehart KL, Wolfe RS.

Component A2 of the methylcoenzyme M methylreductase system from Methanobacterium thermoautotrophicum.

Rouvi�re PE, Escalante-Semerena JC, Wolfe RS.

Formaldehyde activation factor, tetrahydromethanopterin, a coenzyme of methanogenesis.

Escalante-Semerena JC, Leigh JA, Rinehart KL, Wolfe RS.

Tetrahydromethanopterin, a carbon carrier in methanogenesis.

Escalante-Semerena JC, Rinehart KL, Wolfe RS.

Formaldehyde oxidation and methanogenesis.

Escalante-Semerena JC, Wolfe RS.

Nitrate dissimilation under microaerophilic conditions by a magnetic spirillum.

Escalante-Semerena JC, Blakemore RP, Wolfe RS.